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EFFECT OF ROOT-END FILLING MATERIALS ON THE ACTIVITY OF CULTURED PERIODONTAL LIGAMENT FIBROBLASTS AND OSTEOBLASTS

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¾ç¹Ì¿µ/Mi Young Yang ÃÖ±â¿î/¹Îº´¼ø/¹Ú»óÁø/ÃÖÈ£¿µ/Gi woon Choi/Byung Soon Min/Sang Jin Pork/Ho Young Choi

Abstract


The effect of retrograde root-end filling materials(IRM, Super-EBA, Vitremer, MTA)
on human periodontal ligament fibroblasts and osteoblasts was observed. The cell
activities were evaluated by MTT assay, protein assay and alkaline phosphatase activity
examination.
The results as follows ; 1. After 24hrs culture, both E1 cells & PDL fibroblast adding
root-and filling materials were suppressed cell activities but after 48hrs, cell activities
were recovered.
2. Cell activity was lowest in Vitremer followed by IRM, MTA, Super-EBA.
3. Cell activity depression by Vitremer was not concerned with pH changes.
4. Protein synthesis by root-end dilling materials were not significant difference in Both
E1 cell & PDL fibroblasts but protein synthesis were a little increased by Super-IBA.
5. Alkaline phosphatase activity was increased in E1 cell by Super-EBA & MTA but
was not significant differences in E1 cell by IRM & Vitremer. Alkaline phosphatase
activity was a little depressed in PDL fibroblast by Vitremer.
This findings suggest that these root-end filling materials may have important roles
in promotion of PDL healing and consequently may be useful for clinical application in
apical surgery.

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